Enter your oligo sequence in the box and press "Calculate"
Melting Temperature (Tm)  °C 
%GC content
Molecular Weight:  daltons (g/M) 
OD of 1 is equal to  pmol/ml (nM). 

[Original Javascript program by Eugen Buehler,
University of Pittsburgh School of Medicine]


The common Tm formulas for calculating the theoretical Tm of an oligo are:

The simlest formula:

Tm = 4°C  x  (number of G’s and C’s in the primer) + 2°C  x  (number of A’s and T’s in the primer)

Salt-Adjusted Tm Calculation Formula:

Tm= 81.5 + 16.6 x (log10{[Na+] + [K+]}) + 0.41 x (%G+C) – 675/n

Where: n is the number of nucleotides of the oligo
[Na+] and [K+] are the final concentrations of the sodium and potassium ions in the reaction, respectively

Other Tools And Calculators



PCR Guru
WB Guru


Shop for accessories here:



[ Homepage] [Donate] [Tell Friends][Our Button - Link to us] [ Our favorite Links] [Contact Us]

[Scientific eBooks] [ e-Tools for Scientists][ Scientific Photos] [ Journal Club Pal] [ Shop] [ Multimedia] [ Quizzes]
GRE Subject Biochemistry, Cell and Molecular Biology eBooks and Practice Questions]
[ GRE Subject Biochemistry Blog ]
[ PCR (Polymerase Chain Reaction) Troubleshooting, Optimization, Tips and Tricks]
ez-PubMed ][ Rusca Online] [ Index of Named Things in Chemistry and Physics]

[Antibiotics] [ Business eBooks] [ Story eBooks] [ Cloning] [ Chemical Densities] [ Dideoxy Sequencing] [DNA to protein calc]
DNA Microarrays] [Health and Fitness e-Books] [ Scientific Icons] [ Western Blotting (Immunoblotting) ] [ Immunoprecipitation]
OD260 DNA concentration determination calculator ] [ SDS-PAGE] [ Yeast Two-Hybrid] [ RFLP] [ Plasmid Construction]

Multimedia - Flash Animations, Videos and Movies:
Reporter Constructs] [ Screening Oligonucleotide][Phage Display Technology]

Get biology research paper help from domyresearchpaper's experts.